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Unlike the cellular Cbl, the transforming mutants of Cbl were tyrosine-phosphorylated in an adhesion-independent manner and interacted with and activated signalling molecules both in suspended and in adherent cells. Further, the oncogenic forms of Cbl induced anchorage-independent but serum-dependent proliferation of cells. These results support the view that transformation by Cbl results from constitutive activation of integrin-dependent rather than growth factor-dependent signalling events.


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University of Oulu. Jultika University of Oulu repository. Docking proteins p Cas and p C Find Advanced. Abstract Adhesive interactions between cells and extracellular matrix proteins play a vital role in biological processes such as cell proliferation, differentiation and survival. Oulu University Library P. Docking proteins p Cas and p Cbl in integrin and growth factor receptor signalling Saved in:. Ojaniemi, Marja 1. Academic Dissertation to be presented with the assent of the Faculty of Medicine, University of Oulu, for public discussion in Auditorium 12 of the University Hospital of Oulu, on August 10th, , at 12 noon.

Acta Universitatis Ouluensis. D, Medica. Upon stimulation, somatosensory receptors, which are involved in tactile sensitivity, trigger a series of events leading to an accurate perception of the immediate environment and causing an appropriate response to it. Think of a cell as an independent functional unit in the social context of a multicellular organism. Would not the cell's perception of its extracellular surroundings be relevant to establish appropriate connections with other cells, to provide orientation in three-dimensional space, and to participate in coordinated morphogenetic and regenerative processes?

Adhesion receptors ARs fulfill most of these requirements in cells. There is no doubt that ARs are "sticky" molecules, as we briefly mention below, but ARs also contribute signals that allow cells to migrate, proliferate, and survive. ARs act as part of the developmental programs affecting all cells in a given organ or tissue in a timely and coordinated fashion.

ARs generate and transduce important signals to provide accurate spatial information. These signals are essential for cell migration during embryogenesis and growth arrest at defined stages during organogenesis as well as for the vigorous cell proliferation that occurs in regenerating tissues responding to injury. Decades of experimental evidence show that ARs have dual roles. In one, ARs act as glue that holds cells together and attaches cells to the extracellular matrix. But also in their other role they function as molecular sensors, which provide spatial information that profoundly affects key cellular responses.

These cellular responses range from motility to proliferation and survival. As we see below, many investigators have worked to experimentally dissect these two equally important functions of ARs since the mids. Figure Detail Based on their structural features, ARs are conventionally grouped in four superfamilies: the integrins, cadherins, selectins, and immunoglobulin-like cell adhesion molecules Ig-CAM.

Each superfamily consists of numerous members. Notwithstanding their major structural differences and heterogeneous tissue distribution, all ARs share common features Figure 1. Second, all ARs interact through their short cytoplasmic domain with a host of peripheral adaptor proteins, which in turn dynamically connect ARs to the cell's cytoskeleton , mainly with actin filaments.

In essence, we can view adhesion receptors as pillars of reinforced concrete, connecting the foundations of a building the cell to its main frame through grids of metal bars the cytoskeleton. Scientists have learned that genetic or functional inactivation of ARs, particularly the widely expressed integrins and cadherins, leads to severe phenotypes, in which normal embryonic development fails to occur.

These devastating phenotypes are mainly the result of defective cell-cell adhesion in the case of cadherins and defective adhesion of cells to the interstitial matrix for most integrins. In both experimental animals and in patients, the inactivation of ARs is incompatible with life unless the given AR has a restricted tissue distribution. Remarkably, bacterial skin infections in LAD patients do not lead to pus formation because pus results from the accumulation of dead leukocytes at the site of an infection. Now, let us return to our starting assumption, that besides providing adhesion, ARs also convey signals that are important for many essential cellular responses.

How did scientists discover these additional functions?

If ARs were major determinants of the social behavior of cells in multicellular organisms, it would be fair to hypothesize that they have coevolved with metazoans. Is that actually the case? Let us focus on the two main families of ARs, integrins and cadherins, which are engaged in cell-extracellular matrix and cell-cell adhesion, respectively.

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Adhesive Interactions in Normal and Transformed Cells

Indeed, integrins generally appear in early metazoans, such as the sponge Geodia cydonium , although sequences homologous to their conserved extracellular domains are found in prokaryotes Johnson et al. These data suggest that the ligand-binding motifs of integrins were selected early in animal evolution. However, their adhesive function, which entails spanning the plasma membrane and indirectly linking to cytoskeletal elements, is a unique feature in metazoans. The major cadherin families also appear in metazoans, and scientists have learned they are the main molecules responsible for segregating homologous cells away from one another Gumbiner When scientists performed in vitro experiments that mixed cells expressing either cadherin X or Y in a single cell suspension, they observed that the cells readily formed homotypic clusters, meaning that the cadherin X-expressing cells segregated away from the cadherin Y-expressing cells Takeichi Using inductive reasoning, scientists speculated that this phenomenon was the basis for tissue and organ morphogenesis, which is the main distinctive feature of multicellular organisms.

By studying cadherins, scientists proposed this mechanism for tissue and organ morphogenesis long before the responsible coding genes were identified, comparatively analyzed, and manipulated using DNA recombination in model organisms Takeichi Scientists observed for years that when normal cells grow on a substratum made of extracellular matrix proteins, their proliferation is "anchorage-dependent.

What regulates these stop and start signals?

Scientists postulated that ARs delivered signals that influenced the cells' proliferative responses to growth factors. Through careful observations and experiments, they learned that the "start" signals are delivered by integrins and the "stop" signals are mediated by cadherins. Strikingly, both of these responses are completely lost in tumor cells, which show anchorage-independent growth and proliferate freely and endlessly on top of one another with no contact inhibition. Hence, understanding the nature of these AR signals can help us understand how normal cells are transformed into cancerous cells.

The nodes are shape- and colour-coded according to the function of the proteins. Intrinsic components are surrounded by a black frame and associated components by a grey frame. Functional atlas of the integrin adhesome. Nature Cell Biology 9, All rights reserved.

Introduction

Scientists were curious about whether processes regulated by ARs in vivo required intracellular signal transduction or merely the extracellular adhesive functions of ARs. In principle, in the absence of extracellular ligand recognition, there can be no signaling unless the signaling function is completely unrelated to ligand engagement.

Researchers observed that adhesion sites may behave as "hubs" for intracellular signaling pathways when they noted that the bulk of tyrosine phosphorylation within cells under steady-state conditions occurs at adhesion sites. These adhesion sites contained either integrins in areas called focal adhesions or cadherins at adherence junctions Maher et al. Tyrosine phosphorylation is a widely used posttranslational modification in signal transduction cascades that is carried out by enzymes called tyrosine kinases. Could the adhesion receptors themselves be endowed with intrinsic tyrosine kinase activity, and did ligand engagement activate the enzymatic function?

Adhesive Interactions in Normal and Transformed Cells | Yury A. Rovensky | Springer

Scientists ruled out these possibilities by showing that ARs do not possess catalytic domains. In , one research group identified a nonreceptor tyrosine kinase that associated constitutively with integrin-containing adhesion sites and was directly activated by integrin-mediated adhesion. To emphasize its unique subcellular localization, the kinase was named the focal adhesion kinase FAK Parsons et al. Interestingly, upon activation, FAK is able to recruit a host of signaling adaptors, including Grb2 and Sos, which are shared by well-characterized signal transducing receptors.

Thus, the activation of FAK leads to the activation of downstream pathways, which overlap with those triggered by growth factors, and ultimately leads to the onset of DNA replication Juliano ; Parsons et al. Thus, integrins could behave as scaffolds by assembling large molecular networks that connect the plasma membrane to the actin cytoskeleton at sites of adhesion. These scaffolds could produce a local enrichment of signaling adaptors and effectors otherwise shared by other common signal transducing molecules, such as receptor tyrosine kinases RTK.

Recently, scientists performed experiments that strongly corroborated this hypothesis. They performed "in silico" mapping of the "adhesome," which is the molecular network dynamically assembled at focal adhesions upon integrin engagement Zaidel-Bar et al. By examining existing protein interaction databases, the researchers learned that the adhesome contains more than proteins.

These proteins have a relatively small number of key sections in which a scaffolding molecule recruits both a signaling molecule and its downstream target Figure 2. By performing experiments that introduced subtle mutations in the integrin cytoplasmic domains, scientists collected more stringent evidence that integrins indeed carry out two distinct functions in cells. These mutations compromised selected signaling functions without impairing the receptor's overall adhesive function. Both integrin mutants had functional defects that could be specifically ascribed to signaling function impairments.

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